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(Solution) - Mammalian cells growing in culture were labeled with 3H thymidine to

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Mammalian cells growing in culture were labeled with [3H]-thymidine to estimate the rate of DNA synthesis. Te thymidine administered had a speci?c activity of 3000 cpm / pmol. At intervals, samples of culture were taken and acidi?ed to precipitate nucleic acids. Te rate of incorporation of isotope into DNA was 1500 cpm / 106 cells / min. A portion of culture was taken to determine the speci?c activity of the intracellular dTTP pool, which was found to be 600 cpm / pmol.
(a) What fraction of the intracellular dTTP is synthesized from the exogenous precursor?
(b) What is the rate of DNA synthesis, in molecules per minute per cell of thymine nucleotides incorporated into DNA?
(c) How could you determine the speci?c activity of the dTTP pool?

 







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